Journal: Journal of Radiation Research
Article Title: Time-dependent 53BP1 foci kinetics in X-irradiated human hair follicle dermal papilla cells
doi: 10.1093/jrr/rrag005
Figure Lengend Snippet: Representative immunofluorescence images of 53BP1 foci in HFDPCs. Image description: Representative images showing the distribution of 53BP1 foci (red) in human hair follicle dermal papilla cells (HFDPCs) following X-ray irradiation. Focus size classification: small foci: Indicated by white arrows, these represent typical repair sites with diameters consistent with the initial mean of 0.68 ± 0.07 μm. Large foci: Foci with a diameter ≥ 1.0 μm, which become more prominent at later time points and higher doses. Scale bar: The scale bar represents 5 μm.
Article Snippet: The HFDPCs were cultured in a specific growth medium (Follicle Dermal Papilla Cell Growth Medium kit, Cat# C-26502, PromoCell) in an incubator at 37°C with 95% humidity, 20% oxygen and 5% carbon dioxide.
Techniques: Immunofluorescence, Irradiation